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Welcome to gm-csf 

Gentaur - Antibodies, ELISA kits, PCR, Cell culture, Kronos Dio, Bullet Blender

We are committed to providing products that will allow you to maximize your time and money.
For more information on the products we distribute, please click on the left of this page.

 


Recombinant Human Granulocyte Macrophage Colony Stimulating Factor GM CSF

(MGMP-rHuGM-CSF)

Price: 385 Euro/ 300µg

Certificate of Analysis and Data Sheet

Description: mGMP Recombinant Human GM-CSF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477 Dalton. rHuGM-CSF is purified by proprietary chromatographic techniques.

 Source: Escherichia Coli.

 Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation & Packaging: The protein was lyophilized after extensive dialysis against 2mM sodium phosphate buffer pH= 7.4±0.1.

Solubility: The lyophilized rHuGM-CSF is very soluble in water and most aqueous buffers below and above the isoelectric point (pI=4.95).

Stability: Lyophilized mGMPrHuGM-CSF although stable at room temperature, should be stored desiccated below 0°C. Reconstituted rHuGM-CSF is best stored refrigerated at 4°C.

Purity: Greater than 99.0% as determined by:

           (a) Analysis by RP-HPLC (See Figure1).

           (b) Anion-exchange FPLC.

         (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained (See Figure 2).

(Limit of acceptance:ł98.0%. No more than 2% total impurities; no single impurity greater than 1%)

Amino Acid Composition: In total agreement with the expected amino acid composition of native 
human GM-CSF.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser, conforming the sequence of native human GM-CSF. N-terminal methionine has been completely removed enzymatically.

Dimers and aggregates: Less than 1% as determined by silver stained SDS-PAGE gel analysis. 

Biological Activity: mGMPrHuGM-CSF is fully biologically active when compared to standard.The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is 0.1 ng/ml, corresponding to a Specific Activity of 9x106 IU/mg or 2 800 000 IU/ vial of 300 ug

Endotoxin: Less than 0.1 ng/µg (IEU/µg) of mGMPrHuGM-CSF.

Protein content: Protein quantitation was carried out by two independent methods: 

1.   UV spectroscopy at 280 nm using the absorbency value of 0.963 as the extinction

       coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE

       computer analysis program of protein sequences (IntelliGenetics).

2.  Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard. 

Usage: This material is offered by Gentaur for research, laboratory or further manufacturing purposes adn DC culture

 

Cell lines

Hematopoietic cell lines K562, human erythroleukemia cell line (HEL), HL60, TF-1, Jurkat, Daudi, CEM, and ML1 (ATCC, Manassas, VA, USA) were cultured in the presence of 10% fetal bovine serum (FBS) (Euroclone, Milano, Italy) in Iscove's modified Dulbecco's medium (IMDM) or RPMI (Euroclone, Milano Italy) and 5% CO2 in a humidified atmosphere of 5% O2 in air at 37°C. TF-1 required the addition of 10 ng/ml of human granulocyte-macrophage colony-stimulating factor (GM-CSF, Leukomax; Schering-Plough, Basel, Switzerland).

 

 

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Last modified: 06/25/09